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Biosynthesis of podophyllotoxin in Linum album cell cultures.
- Source :
- Planta: An International Journal of Plant Biology; Oct2002, Vol. 215 Issue 6, p1031-1039, 9p
- Publication Year :
- 2002
-
Abstract
- Cell cultures of Linum album Kotschy ex Boiss. (Linaceae) showing high accumulation of the lignan podophyllotoxin (PTOX) were established. Enzymological studies revealed highest activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, 4-hydroxycinnamate:CoA ligase and cinnamoyl-CoA:NADP oxidoreductase immediately prior to PTOX accumulation. To investigate PTOX biosynthesis, feeding experiments were performed with [2-<superscript>13</superscript>C]3′,4′-dimethoxycinnamic acid, [2-<superscript>13</superscript>C]3′,4′-methylenedioxycinnamic acid (MDCA), [2-<superscript>13</superscript>C]3′,4′,5′-trimethoxycinnamic acid, [2-<superscript>13</superscript>C]sinapic acid, [2-<superscript>13</superscript>C]- and [2,3-<superscript>13</superscript>C<subscript>2</subscript>]ferulic acid. Analysis of the metabolites by HPLC coupled to tandem mass spectrometry revealed incorporation of label from ferulic acid into PTOX and deoxypodophyllotoxin (DOP). In addition, MDCA was also unambiguously incorporated intact into PTOX. These observations suggest that in L. album both ferulic acid and methylenedioxy-substituted cinnamic acid can be incorporated into lignans. Furthermore, it appears that, in this species, the hydroxylation of DOP is a rate-limiting point in the pathway leading to PTOX. Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/wo.1007/s00425-002-0834-1. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOSYNTHESIS
PODOPHYLLOTOXIN
LINUM
OXIDOREDUCTASES
AMINO acids
BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 00320935
- Volume :
- 215
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Planta: An International Journal of Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 16126275
- Full Text :
- https://doi.org/10.1007/s00425-002-0834-1