Tentative Method of Sugar Estimation in Laminae and its Application to Hevea brasiliensis.

The preparation of sugar-containing extracts from the laminae of Hevea brasiliensis is described and a one dimensional descending chromatographic method of estimating the individual sugars is given. The chromatograms are developed for 48 hours, dipped in silver nitrate, and the spots cut out and weighed; the amount of sugar or inositol present in each spot is obtained by reference to a calibration curve prepared from standard amounts of sugars developed under similar conditions. The method is simple and specific and has been applied to the preliminary investigation of the effect of varied mineral nutrient status on the sugar content of the limanae of Hevea; these results are described briefly. [ABSTRACT FROM AUTHOR]