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Activation of human lymphocytes by supernatants from human thymic epithelium.

Authors :
Goust, J. M.
Vesole, D. H.
Fudenberg, H. H.
Source :
Clinical & Experimental Immunology; Nov1979, Vol. 38 Issue 2, p348-355, 8p
Publication Year :
1979

Abstract

Supernatants from human thymic epithelial cells (TS) were found to have a mitogenic effect on cultured human peripheral blood mononuclear cells and to potentiate their responses to lectins. This was not observed with culture supernatants from the human cell lines AV-3 and HeLa or from the murine cell line L-929. The maximum potentiating effects were observed with pokeweed mitogen (PWM) and phytohaemagglutinin (PHA), whereas the response to concanavalin A (Con A) was only slightly enhanced. TS also potentiated the mixed lymphocyte culture (MLC) response of normal T cells and thymocytes cultured with mitomycin C-treated B lymphoid cell lines. The mitogenic effect of TS was time-dependent and paralleled the appearance of lymphoid colonies in semi-solid agar. Chromatographical separation of concentrated serum-free TS on Sephadex G-100 yielded an active fraction of molecular weight 15,000-25,000 which had all the activities of unseparated TS. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00099104
Volume :
38
Issue :
2
Database :
Complementary Index
Journal :
Clinical & Experimental Immunology
Publication Type :
Academic Journal
Accession number :
16108370