Remaining uncertainties in the Pneumatic method.

No gas source, no problem: proximity to pre-existing embolism and segmentation affect embolism spreading in angiosperm xylem by gas diffusion. However, our micro-CT images showed heterogeneity of embolism along the branch sample under given dehydration stages (See Methods S2), and the embolism level did not consistently decrease from the cut end to the distal end (Fig. However, vessels in proximity to air sources (e.g. pre-existing embolism, cut-open vessels) are reported to be more prone to embolism (Brodersen I et al i ., [2]; Guan I et al i ., [6]). Keywords: climate change; drought resistance; embolism; micro-CT; plant hydraulics; vulnerability curves EN climate change drought resistance embolism micro-CT plant hydraulics vulnerability curves 384 391 8 12/22/22 20230115 NES 230115 A reply to Brum et al. (2023) 'Reconciling discrepancies in measurements of vulnerability to... The integrity of the plant water transport system is crucial for plant performance and survival (Rowland I et al i ., [14]). [Extracted from the article]