The effect of COVID-19 lockdown on mental health, gut microbiota composition and serum cortisol levels.

The aim of this study was to assess changes in mental health, gut microbiota composition, and stress marker serum cortisol due to COVID-19 lockdown in asymptomatic individuals. Healthy adults participated in anthropometric measurements, blood and stool sample collection pre-lockdown and postlockdown (n=38, 63.2% females), lifestyle and psychological questionnaires were included in pre-lockdown measurement and lockdown survey (n=46, 67.4% females). Subjects reported significantly higher body dissatisfaction (p=0.007) and anxiety (p=0.002), and significantly lower positive affect (p=0.001) during lockdown compared with pre-lockdown. According to perceived stress, 51.6% of females and 20% of males experienced moderate to high stress. This was reflected in serum cortisol levels that significantly increased only in females (p=0.006) post-lockdown and correlated with perceived stress (p=0.037) and anxiety (p=0.031). In addition to psychological measures, changes in gut microbiota composition were observed. Gut microbial alpha diversity significantly decreased (p=0.033), whereas relative abundance of Proteobacteria significantly increased (p=0.043) post-lockdown. Depression during lockdown was moderately positively correlated with changes in Bacteroidetes abundance (p=0.015) and negatively with changes in Firmicutes abundance (p=0.008). Alistipes abundance post-lockdown was moderately positively correlated with anxiety (p=0.004) and negative affect (p=0.005) during lockdown. Despite a small sample size and not being able to perform objective measurements during lockdown, the results confirm the effect of lockdown on mental health and gut microbiota composition that could have a great impact on our health (ClinicalTrials identifier: NCT04347213). [ABSTRACT FROM AUTHOR]