Inhibitory effect of Sparassis latifolia polysaccharides on cariogenic bacteria as studied in-vitro simulated oral processing.

Growth inhibition of Streptococcus mutans (SM), Streptococcus salivarius (SS1), and Streptococcus sanguinis (SS2) were determined at different Sparassis latifolia polysaccharides (SLPs) concentrations (2%, 4%, 8%, 16%) mixed with artificial saliva (AS). Metabolomics was determined by the action of 4% SLPs and AS on each bacterium to obtain the bacteriostatic activity of SLPs on oral processing. The results showed that the 16% mixture could inhibit the growth of the oral bacteria S. mutans, S. salivarius, and S. sanguinis. The inhibitory effect on oral bacteria decreased with decreasing polysaccharide concentration. The metabolic pathways and metabolites analyses screened for active inhibitory substances against oral bacteria. These results implied that SLPs are potential new material for developing oral functional products. [ABSTRACT FROM AUTHOR]