IN-VITRO ANTI-CANCER ACTIVITY AND CYTOTOXICITY OF MEMBRANE EXTRACELLULAR VESICLES ISOLATED FROM STAPHYLOCOCCUS AUREUS CLINICAL ISOLATES.

Like Gram negative, Gram positive bacteria release nano-sized vesicles into the extracellular environment in a constitutive manner. These bacterial extracellular vesicles (EVs) are spherical bilayered proteolipids enriched with bioactive proteins, lipids, nucleic acids and virulence factors. Recent progress in this field supports the critical assumption of the anticancer activity of these extracellular vesicles. Using MTT assay, Staphylococcus aureus derived extracellular vesicles anticancer activity was evaluated. Results showed no noticeable percentages of cytotoxicity (CT%) for EVs against normal HBL-100 cells, since the CT% ranged from 2% to 10.67% at higher concentrations. While, there was higher cytotoxicity against breast cancer cells MCF-7 ranged from 27.29 to 58.64% for AMJ13 cells and 8.33% to 79.33% for MCF-7 after Evs treatment. The cytotoxicity assay analysis showed that IC50 values of EVs (19.71 µg MCF-7, 634 µg HBL-100). [ABSTRACT FROM AUTHOR]