Spectrophotometric determination of chlordiazepoxide with 2-(chloromethyl)-5-methyl-1H-benzo [d] imidazole reagent.

A fast, and accurate spectrophotometric method for the determination of chlordiazepoxide (CDE) in its pure form and the pharmaceutical preparation, This method is based on a complex formation between CDE and the DBM reagent to give a complex that gave the maximum absorption at the wavelength of 290 nm. The optimum conditions required for the formation of the complex were found (reagent concentration, time, temperature, and the medium of the reaction). The linearity of the method ranged between 35-70 µg/ml, the molar absorptivity of 8.214×10³ L/mol.cm and the Sandell's index was 0.03649 µg/cm², the detection limit was 2.2802 µg/ml and the quantification limit was 6.9097 µg/ml. Rec% was between 98.859-102.099% and the RSD% was between 0.04447-0.21407%. This method was found to be accurate, precious and has been successfully applied to quantify CDE in pharmaceutical form Libroxide. [ABSTRACT FROM AUTHOR]