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A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology.
- Source :
- Histochemistry & Cell Biology; Nov2022, Vol. 158 Issue 5, p463-469, 7p
- Publication Year :
- 2022
-
Abstract
- The anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee's osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states. [ABSTRACT FROM AUTHOR]
- Subjects :
- ETIOLOGY of diseases
X-ray fluorescence
CARTILAGE
EXTRACELLULAR matrix
STRONTIUM
Subjects
Details
- Language :
- English
- ISSN :
- 09486143
- Volume :
- 158
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Histochemistry & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 160028866
- Full Text :
- https://doi.org/10.1007/s00418-022-02135-8