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Elastic properties and shape of the Piezo dome underlying its mechanosensory function.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 10/4/2022, Vol. 119 Issue 40, p1-8, 8p
- Publication Year :
- 2022
-
Abstract
- We show in the companion paper that the free membrane shape of lipid bilayer vesicles containing the mechanosensitive ion channel Piezo can be predicted, with no free parameters, from membrane elasticity theory together with measurements of the protein geometry and vesicle size [C. A. Haselwandter, Y. R. Guo, Z. Fu, R. MacKinnon, Proc. Natl. Acad. Sci. U.S.A., 10.1073/pnas.2208027119 (2022)]. Here we use these results to determine the force that the Piezo dome exerts on the free membrane and hence, that the free membrane exerts on the Piezo dome, for a range of vesicle sizes. From vesicle shape measurements alone, we thus obtain a force–distortion relationship for the Piezo dome, from which we deduce the Piezo dome’s intrinsic radius of curvature, 42 ± 12 nm, and bending stiffness, 18 ± 2:1 k<subscript>B</subscript>T, in freestanding lipid bilayer membranes mimicking cell membranes. Applying these estimates to a spherical cap model of Piezo embedded in a lipid bilayer, we suggest that Piezo’s intrinsic curvature, surrounding membrane footprint, small stiffness, and large area are the key properties of Piezo that give rise to low-threshold, high-sensitivity mechanical gating. [ABSTRACT FROM AUTHOR]
- Subjects :
- BILAYER lipid membranes
ELASTICITY
CELL membranes
ION channels
SHAPE measurement
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 119
- Issue :
- 40
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 159587946
- Full Text :
- https://doi.org/10.1073/pnas.2208034119