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EGTA induces the synthesis in <em>Escherichia coli</em> of three proteins that cross-react with calmodulin antibodies.

Authors :
Laoudj, Dalila
Andersen, Catherine L.
Bras, Ana
Goldberg, Martin
Jacq, Annick
Holland, I. Barry
Source :
Molecular Microbiology; Aug1994, Vol. 13 Issue 3, p445-457, 13p, 7 Black and White Photographs, 1 Chart, 1 Graph
Publication Year :
1994

Abstract

&lt;em&gt;Escherichia coli&lt;/em&gt; mutants, (&lt;em&gt;verA, dilA&lt;/em&gt;) specifically resistant to the Ca&lt;superscript&gt;2+&lt;/superscript&gt; channel inhibitors verapamil and diltiazem, respectively, are hypersensitive to EGTA and BAPTA. We have shown, using 1-D and 2-D gel electrophoresis, that the synthesis of at least 25 polypeptides in the mutants was enhanced by treatment with Ca&lt;superscript&gt;2+&lt;/superscript&gt; chelators and the synthesis of at least 11 polypeptides was repressed. This pattern of induction was not observed in heat- or SDS-treated cells and therefore does not appear to be a general stress response. The majority of the induced proteins are low molecular weight, extremely heat stable and acidic, characteristic properties of caimodulin. Moreover, of the major induced species, three with apparent molecular masses of 12, 18, and 34kDa all cross-reacted with polydonal and monoclonal anti-bodies to eukaryote calmodulins or calerythrin, a heat-resistant Ca&lt;superscript&gt;2+&lt;/superscript&gt;-binding protein from &lt;em&gt;Saccharo-polyspora erythraea&lt;/em&gt;. The &lt;em&gt;verA&lt;/em&gt;, &lt;em&gt;dilA&lt;/em&gt; mutants. In being hypersensitive to EGTA and to the Ca&lt;superscript&gt;2+&lt;/superscript&gt; ionophore A23187 + Ca&lt;superscript&gt;2+&lt;/superscript&gt;, may be defective in the regulation of the level of free intracellular Ca&lt;superscript&gt;2+&lt;/superscript&gt;. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0950382X
Volume :
13
Issue :
3
Database :
Complementary Index
Journal :
Molecular Microbiology
Publication Type :
Academic Journal
Accession number :
15945615
Full Text :
https://doi.org/10.1111/j.1365-2958.1994.tb00439.x