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Tryptophan capped gold-aryl nanoparticles for energy transfer study with SARS-CoV-2 spike proteins.
- Source :
- Soft Materials; 2022, Vol. 20 Issue 4, p405-413, 9p
- Publication Year :
- 2022
-
Abstract
- Current research on coronavirus 2 disease (SARS-CoV-2) has been focused on developing reliable gold nanomaterials and methods for the detection of viral spike protein (S-proteins). Here, we describe the fluorescence quenching of spike proteins in the presence of robust gold-aryl nanoparticles. The obtained results demonstrated a strong binding affinity between S-proteins and tryptophan immobilized on gold-carbon nanoparticles (Trp-AuNPs) based on the binding constant value of 11.098 M<superscript>−1</superscript>. Förster non-radiative energy transfer (FRET) calculations showed an average binding distance of r = 3.06 nm and a critical energy transfer distance of R<subscript>0</subscript> = 2.5 nm. Time-resolved fluorescence studies of conjugated S-proteins onto Trp-AuNPs revealed a short fluorescence lifetime and dynamic quenching. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1539445X
- Volume :
- 20
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Soft Materials
- Publication Type :
- Academic Journal
- Accession number :
- 158963255
- Full Text :
- https://doi.org/10.1080/1539445X.2022.2053543