Analysis of Thioredoxins and Glutaredoxins in Soybean: Evidence of Translational Regulation under Water Restriction.

The four combined treatments were nodulated (N) water-restricted (WR) plants (N+WR), nodulated well-watered (WW) plants (N+WW), non-nodulated (NN) water-restricted plants (NN+WR), and non-nodulated well-watered plants (NN+WW). Graph: Figure 4 Differentially expressed gene (DEG) analysis and GmTrx and GmGrx expression profiles in nodulated (N) and water-restricted (WR) plants with respect to well-watered (WW) and non-nodulated (NN) plants. Graph: Figure 6 String analysis [[41]] derived protein-protein interaction network obtained for the differentially expressed genes down-regulated at the TOTAL+PAR level in nodulated and water-restricted plants with respect to non-nodulated and water-restricted plants (N+WR vs. To gain more insight into the functionality of the DEGs, we analyzed the distribution and localization of the most frequent I GmTrx i and I GmGrx i CREs (stress response: ARE and WUN motif; site-binding-related: MYC and MYB; hormone response: CGTCA, TGACG, TCA, and ABRE; and light response: TCT and Box IV) in the promoter regions of the DEGs. From the total detected I GmTrx i and I GmGrx i genes in our RNA-seq experiment, 10% and 20%, respectively, were differentially expressed in nodulated and water-restricted plants, suggesting a relevant role of these proteins in the plant response to water-deficit stress in nodulation conditions. [Extracted from the article]