Assessing Cellular Uptake of Exogenous Coenzyme Q 10 into Human Skin Cells by X-ray Fluorescence Imaging.

Over the full range of all 100 cells, a mean value of 77.03 ± 21.48 fg iodine/cell ( 2.6 × 10 SP -4 sp ± 0.73 ng Q SB 10 sb /cell) was calculated, with a median value of 49.13 fg iodine/cell ( 1.66 × 10 SP -4 sp ng Q SB 10 sb /cell). Control measurements using free iodine, experiments of I SB 2 sb -Q SB 10 sb bioavailability via LC-MS/MS as well as the scans around the cell pellets clearly indicate that the measured iodine masses can only be related to the I SB 2 sb -Q SB 10 sb . (a) 26.7 ng total iodine mass in cell pellet from donor 1, (b) 28.8 ng total iodine mass in cell pellet from donor 2, (c) 27.4 ng total iodine mass in cell pellet from donor 3, (d) no iodine signal in untreated cell sample. The total reconstructed iodine masses in the capillaries were 26.7 ± 4.0 ng, 28.8 ± 4.3 ng and 27.4 ± 4.1 ng for donor 1 (600,000 cells), 2 (600,000 cells) and 3 (400,000 cells), respectively, whereas no iodine could be detected in the untreated cell sample (750,000 cells). [Extracted from the article]