Reverse and Forward Electron Flow-Induced H 2 O 2 Formation Is Decreased in α-Ketoglutarate Dehydrogenase (α-KGDH) Subunit (E2 or E3) Heterozygote Knock Out Animals.

This effect could be attributed to the ADP-dependent depolarization of I i SB m sb , to the stimulatory effect of ADP on the KGDHc [[66]], and to ADP-dependent disinhibition of KGDHC by succinyl-CoA due to the participation of ADP in the succinyl-thiokinase reaction. Reverse and Forward Electron Flow-Induced H 2 O 2 Formation Is Decreased in -Ketoglutarate Dehydrogenase ( -KGDH) Subunit (E2 or E3) Heterozygote Knock Out Animals Taking into account that ( I i i ) KGDHc is a TCA cycle enzyme with a considerable flux control coefficient [[6], [27]], ( I ii i ) KGDHc is capable of generating a high amount of ROS [[23], [25]], and ( I iii i ) mitochondrial ROS generation is often involved in the pathogeneses of neurodegenerative diseases [[28]], it is not surprising that numerous diseases have been associated with impairments of the KGDHc subunits [[29], [31], [33], [35]]. H 2 O 2 Production in Mitochondria Respiring on Succinate and -Glycerophosphate In the absence of ADP, succinate-supported mitochondria exhibited an extremely high rate of ROS production which can be attributed to the reverse electron transfer (RET) [[45], [57]]. Keywords: KGDHc; -ketoglutarate dehydrogenase complex; OGDHc; oxoglutarate dehydrogenase complex; ischemia-reperfusion; antioxidant systems; DLD; DLST; mitochondria; reactive oxygen species; reverse electron transfer; RET; ROS; cellular respiration; transgenic animal; succinate; -glycerophosphate EN KGDHc -ketoglutarate dehydrogenase complex OGDHc oxoglutarate dehydrogenase complex ischemia-reperfusion antioxidant systems DLD DLST mitochondria reactive oxygen species reverse electron transfer RET ROS cellular respiration transgenic animal succinate -glycerophosphate N.PAG N.PAG 19 08/29/22 20220801 NES 220801 1. [Extracted from the article]