Back to Search Start Over

RSPO3 is a novel contraction-inducible factor identified in an "in vitro exercise model" using primary human myotubes.

Authors :
Takahashi, Tadahisa
Li, Yuqing
Chen, Weijian
Nyasha, Mazvita R.
Ogawa, Kazumi
Suzuki, Kazuaki
Koide, Masashi
Hagiwara, Yoshihiro
Itoi, Eiji
Aizawa, Toshimi
Tsuchiya, Masahiro
Suzuki, Naoki
Aoki, Masashi
Kanzaki, Makoto
Source :
Scientific Reports; 8/22/2022, Vol. 12 Issue 1, p1-13, 13p
Publication Year :
2022

Abstract

The physiological significance of skeletal muscle as a secretory organ is now well known but we can only speculate as to the existence of as-yet-unidentified myokines, especially those upregulated in response to muscle contractile activity. We first attempted to establish an "insert-chamber based in vitro exercise model" allowing the miniature but high cell-density culture state enabling highly developed contractile human myotubes to be readily obtained by applying electric pulse stimulation (EPS). By employing this in vitro exercise model, we identified R-spondin 3 (RSPO3) as a novel contraction-inducible myokine produced by cultured human myotubes. Contraction-dependent muscular RSPO3 mRNA upregulation was confirmed in skeletal muscles of mice subjected to sciatic nerve mediated in situ contraction as well as those of mice after 2 h of running. Pharmacological in vitro experiments demonstrated a relatively high concentration of metformin (millimolar range) to suppress the contraction-inducible mRNA upregulation of human myokines including RSPO3, interleukin (IL)-6, IL-8 and CXCL1. Our data also suggest human RSPO3 to be a paracrine factor that may positively participate in the myogenesis processes of myoblasts and satellite cells. Thus, the "insert chamber-based in vitro exercise model" is a potentially valuable research tool for investigating contraction-inducible biological responses of human myotubes usually exhibiting poorer contractility development even in the setting of EPS treatment. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20452322
Volume :
12
Issue :
1
Database :
Complementary Index
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
158694197
Full Text :
https://doi.org/10.1038/s41598-022-18190-z