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Characterization and Expression of the Human T Cell Receptor-T3 Complex by Monoclonal Antibody F101.01.
- Source :
- Scandinavian Journal of Immunology; Jun1988, Vol. 27 Issue 6, p685-696, 12p
- Publication Year :
- 1988
-
Abstract
- A murine monoclonal antibody (MoAb) F101.01 reacting with the T cell receptor (TCR)-T3 complex is presented. Immunobistological studies showed that F101.01 specifically stains T-zone lymphocytes in lymph nodes, tonsils and splenic tissue. Two-colour immunofluorescence and flow cytometry demonstrated co-expression of the antigen defined by F101.01 and the pan-T cell antigens defined by CD2. CD3. CD5, and CD7 antibodies. Cells stained with CD4 and CD8 antibodies were both included in the F101.01-positive population, whereas CD16-positive natural killer cells (NK). B cells (CD19 and CD21)), and myeloid cells (CD 13 and CD.13) were excluded. The target antigen of F101.01 co-modulated with the CD3-defined antigen (T3) and the TCR recognized by the MoAb WT-31. CD3 antibody and WT-31 both blocked binding of F101.01, F101.01 precipitated the TCR-T3 complex From lysates of <superscript>125</superscript>M-labelled peripheral blood mononuclear cells (PBMC) and HPB-ALL, when the lysate was prepared with a detergent (digitonin) that conserves the TCR-T3 complex, FACS analysis of T cells from a patient with a T cell immunodeficiency demonstrated that A-TCS- 1-CD3<superscript>+</superscript> CD4<superscript>+</superscript> and δ-TCS-1-CD3<superscript>+</superscript> CD8<superscript>+</superscript> cells were brightly F101.01<superscript>+</superscript>, whereas a large subpopulation of δ-TCS-l+CD3+CD4-CD8 cells were weakly F101.01, We conclude that F101.01 recognizes a conformational epitope of the TCR-H complex and that it reacts with the αβ TCR-T3 and the γ <superscript>δ</superscript> TCR-T3 complexes with different intensities. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
IMMUNOGLOBULINS
T cells
IMMUNITY
KILLER cells
T cell receptors
Subjects
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 27
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15857018
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1988.tb02402.x