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Simple and Sensitive Detection of Bacterial Hydrogen Sulfide Production Using a Paper-Based Colorimetric Assay.
- Source :
- Sensors (14248220); Aug2022, Vol. 22 Issue 15, p5928-N.PAG, 10p
- Publication Year :
- 2022
-
Abstract
- Hydrogen sulfide (H<subscript>2</subscript>S) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify H<subscript>2</subscript>S produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial H<subscript>2</subscript>S utilizing silver/Nafion/polyvinylpyrrolidone membrane and a 96-well microplate. This H<subscript>2</subscript>S-sensing paper showed a good sensitivity (8.27 blue channel intensity/μM H<subscript>2</subscript>S, R<superscript>2</superscript> = 0.9996), which was higher than that of lead acetate paper (6.05 blue channel intensity/μM H<subscript>2</subscript>S, R<superscript>2</superscript> = 0.9959). We analyzed the difference in H<subscript>2</subscript>S concentration released from four kinds of oral bacteria (Eikenella corrodens, Streptococcus sobrinus, Streptococcus mutans, and Lactobacillus casei). Finally, the H<subscript>2</subscript>S level in Eikenella corrodens while varying the concentration of cysteine and treatment time was quantified. This paper-based colorimetric assay can be utilized as a simple and effective tool for in vitro screening of H<subscript>2</subscript>S-producing ability of many bacteria as well as salivary H<subscript>2</subscript>S analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 22
- Issue :
- 15
- Database :
- Complementary Index
- Journal :
- Sensors (14248220)
- Publication Type :
- Academic Journal
- Accession number :
- 158550479
- Full Text :
- https://doi.org/10.3390/s22155928