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Structural changes of carbohydrate chains of human thyroglobulin accompanying malignant transformations of thyroid glands.
- Source :
- European Journal of Biochemistry; 8/15/84, Vol. 143 Issue 1, p133-144, 12p
- Publication Year :
- 1984
-
Abstract
- Carbohydrate moieties were released from human thyroglobulins prepared from normal and transformed, thyroid tissues by hydrazinolysis. The oligosaccharides thus prepared were fractionated by DEAE-cellulose, Bio-Gel P-4, concanavalin-A - Sepharose and Ricinus-communis-agglutinin- Sepharose columns and were characterized by exo-glycosidase and endo-glycosidase digestions, methylation analysis and 400-MHz <superscript>1</superscript>H-NMR spectroscopy. These studies showed that the alterations accompanying malignancy occurred in complex type carbohydrate chains and exhibited the following structural features: (a) a complex-type carbohydrate chain containing I phosphate in a diester linkage was present in all the malignant thyroglobulins. (b) Asialo-carbohydrate chains with biosynthetically incomplete structures were found in all of the malignant thyroglobulins. (c) Carbohydrate chains of higher molecular mass, which have repeating Gal-GlcNAc disaccharides and peripheral α-fucosyl residues were present in metastatic papillary carcinoma. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 143
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15832234
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1984.tb08352.x