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Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay.
- Source :
- Analytical & Bioanalytical Chemistry; Aug2022, Vol. 414 Issue 20, p6139-6147, 9p
- Publication Year :
- 2022
-
Abstract
- Telomerase is a promising diagnostic and prognostic biomarker for cancers. Sensitive, simple, and reliable telomerase activity detection is vital for cancer diagnosis. Herein, we developed an ultrasensitive visualized assay for telomerase activity that combined the exponential amplification reaction (EXPAR) and lateral flow assay for easy and quick signal readout, which we termed as a lateral flow readout-EXPAR (LFR-EXPAR) assay. In the LFR-EXPAR assay, telomerase elongation products initiate the exponential amplification reaction, the generated trigger hybridizes with the reporter to form the recognition site of the nicking enzyme, and the nicking enzyme cuts the reporter strand. The degradation of the reporter can be detected with a universal lateral flow dipstick and read out with the naked eye. After conducting a series of proof-of-concept investigations, the LFR-EXPAR assay was found to achieve a sensitivity comparable to that of a TRAP (telomere repeat amplification protocol) assay. The LFR-EXPAR assay can be used to realize ultrasensitive and point-of-care detection of telomerase without requiring specialized instruments, holding great promise for early cancer diagnosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- AMPLIFICATION reactions
TELOMERASE
CANCER diagnosis
EARLY diagnosis
TELOMERES
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 414
- Issue :
- 20
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 158164164
- Full Text :
- https://doi.org/10.1007/s00216-022-04179-0