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RuSiO2@Ag Core–Shell Nanoparticles for Plasmon Resonance Energy Transfer-Based Electrochemiluminescence Sensing of Glucose and Adenosine Triphosphate.
- Source :
- ACS Applied Nano Materials; 7/22/2022, Vol. 5 Issue 7, p9996-10002, 7p
- Publication Year :
- 2022
-
Abstract
- Convenient and fast approaches for sensitive monitoring of different biomolecules are of vital importance for obtaining insights into biological processes and related diseases. Herein, silica nanospheres encapsulated with Ru-(bpy)<subscript>3</subscript><superscript>2+</superscript> (RuSiO<subscript>2</subscript>) were uniformly coated with silver nanoparticles (AgNPs) through the in-situ reduction method to form RuSiO<subscript>2</subscript>@Ag core–shell nanoparticles, which were employed to fabricate a versatile electrochemiluminescence (ECL) sensing system for the assay of glucose (Glu) and adenosine triphosphate (ATP) on the basis of ECL resonance energy transfer (ECL-RET). RuSiO<subscript>2</subscript>@Ag core–shell nanoparticles showed high RET efficiency due to the combination of the donor (RuSiO<subscript>2</subscript>)–acceptor (AgNPs) pair into one nanostructure, causing a significantly quenched ECL signal of RuSiO<subscript>2</subscript>. Afterward, the Glu oxidase immobilized on RuSiO<subscript>2</subscript>@Ag by DNA complementation naturally catalyzed the oxidation of Glu to produce H<subscript>2</subscript>O<subscript>2</subscript>, which then triggered the etching of AgNPs. Consequently, the remarkably recovered ECL signal was further used to specifically detect Glu and achieved a limit of detection of 0.15 μM. ATP was chosen as a model molecule to verify the detecting ability of the sensor and achieved femtomolar-level results. The versatile ECL sensing system based on enzymatic oxydate-triggered etching and RET strategies hold potential promise for the convenient and sensitive detection of multiple types of biomolecules. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 25740970
- Volume :
- 5
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- ACS Applied Nano Materials
- Publication Type :
- Academic Journal
- Accession number :
- 158136641
- Full Text :
- https://doi.org/10.1021/acsanm.2c02415