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Small-Angle X-Ray Studies on the Structure of 16-S Ribosomal RNA and of a Complex of Ribosomal Protein S4 and 16-S Ribosomal RNA from <em>Escherichia coli</em>.
- Source :
- European Journal of Biochemistry; Nov75 Part 1, Vol. 59 Issue 1, p63-71, 9p
- Publication Year :
- 1975
-
Abstract
- 16-S ribosomal RNA and a complex of ribosomal protein S4 and 16-S rRNA were studied in solution by small-angle X-ray scattering. Concentration series of the 16-S rRNA and the S4 · 16-S-rRNA complex were measured in 37.5 mM Tris-HCl buffer pH 7.4 at 5°C. The following data were determined. The radii of gyration for the 16-S rRNA and S4 · 16S-rRNA complex were R = 17.6 ± 0.4 nm and 17.5 ± 0.6 nm, respectively. The two respective values of the radii of gyration of the cross-section were R<subscript>q.1</subscript> = 8.42 ± 0.1 nm and 8.33 ± 0.3 nm, and R<subscript>q.2</subscript> = 0.988 ± 0.03 nm and 0.996 ± 0.03 nm. The largest diameters of the 16-S RNA and S4 · 16-S-RNA complex were L = 61.8 ± 1 nm and 60.0 ± 1 nm, respectively. Volumes of V = 1570 ± 60 nm³ were found for both particles. In the Tris buffer used, no significant differences were found between the scattering curves of 16-S rRNA and the S4 · 16-S-rRNA complex. A model equivalent in scattering properties to the RNA and complex is a flat elliptical cylinder with the following dimensions: large axis 61.7 nm, small axis 35.4 nm and height 2 nm. The theoretical scattering curve fits the experimental one as long as the shape of the measured curve is due to the overall shape of the particle. A model equivalent in scattering over the whole measured angular range is one built up from a large numbe of spheres that simulate the known substructure of the RNA. The outer dimensions of this model correspond to those of the flat elliptical cylinder. [ABSTRACT FROM AUTHOR]
- Subjects :
- RNA
RIBOSOMES
ESCHERICHIA coli
X-ray scattering
MESSENGER RNA
NUCLEIC acids
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 59
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15807180
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1975.tb02425.x