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Partial freezing of rat livers extends preservation time by 5-fold.

Authors :
Tessier, Shannon N.
de Vries, Reinier J.
Pendexter, Casie A.
Cronin, Stephanie E. J.
Ozer, Sinan
Hafiz, Ehab O. A.
Raigani, Siavash
Oliveira-Costa, Joao Paulo
Wilks, Benjamin T.
Lopera Higuita, Manuela
van Gulik, Thomas M.
Usta, Osman Berk
Stott, Shannon L.
Yeh, Heidi
Yarmush, Martin L.
Uygun, Korkut
Toner, Mehmet
Source :
Nature Communications; 7/15/2022, Vol. 13 Issue 1, p1-13, 13p
Publication Year :
2022

Abstract

The limited preservation duration of organs has contributed to the shortage of organs for transplantation. Recently, a tripling of the storage duration was achieved with supercooling, which relies on temperatures between −4 and −6 °C. However, to achieve deeper metabolic stasis, lower temperatures are required. Inspired by freeze-tolerant animals, we entered high-subzero temperatures (−10 to −15 °C) using ice nucleators to control ice and cryoprotective agents (CPAs) to maintain an unfrozen liquid fraction. We present this approach, termed partial freezing, by testing gradual (un)loading and different CPAs, holding temperatures, and storage durations. Results indicate that propylene glycol outperforms glycerol and injury is largely influenced by storage temperatures. Subsequently, we demonstrate that machine perfusion enhancements improve the recovery of livers after freezing. Ultimately, livers that were partially frozen for 5-fold longer showed favorable outcomes as compared to viable controls, although frozen livers had lower cumulative bile and higher liver enzymes. The limited preservation duration of donor organs is a big problem. Here the authors report a method for the freezing of whole rat livers at temperatures between −10 °C to −15 °C for up to 5 days, based on freeze-tolerant wood frogs, and term this partial freezing. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
158020409
Full Text :
https://doi.org/10.1038/s41467-022-31490-2