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Glycoproteins Containing Peanut-Agglutinin Receptors from Human-Peripheral- Blood T-Lymphocyte Plasma Membranes.
- Source :
- European Journal of Biochemistry; 12/15/81, Vol. 121 Issue 1, p237-241, 5p
- Publication Year :
- 1981
-
Abstract
- A glycoprotein fraction possessing peanut agglutinin receptors has been isolated from detergent extracts of neuraminidase-treated human peripheral blood T-lymphocyte plasma membranes with affinity matrices comprising the peanut agglutinin co-valently immobilised on Sepharose 4B. This fraction could be specifically eluted from affinity columns using buffer solutions supplemented with 0.2 M D-galactose and was shown, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (0.1 %), to contain four major glycoprotein components with apparent molecular weights of 2000003 190000, 110000 and 95000. It is suggested, from the observed reactivity of these glycoproteins with various lectins in double-diffusion experiments, that they possess both O-glycosidically and N-glycosidically linked carbohydrates. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 121
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15800744
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1981.tb06454.x