Tryptic Digestion of Native Small-Intestinal Sucrase · Isomaltase Complex: Isolation of the Sucrase Subunit.

Limited tryptic digestion of native sucrase. isomaltase complex produced a more rapid destruction of isomaltase activity than sucrase activity. It was possible to isolate a partially fragmented sucrase subunit in high yields with a specific activity twice that of the native complex. Amino acid and carbohydrate analyses are reported and compared with the results obtained for sucrase isomaltase complex and isomaltase subunit obtained by a different method. [ABSTRACT FROM AUTHOR]