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MyoBio: An Automated Bioreactor System Technology for Standardized Perfusion-Decellularization of Whole Skeletal Muscle.
- Source :
- IEEE Transactions on Biomedical Engineering; Jul2022, Vol. 69 Issue 7, p2305-2313, 9p
- Publication Year :
- 2022
-
Abstract
- Objective: Decellularizing solid organs is a promising top-down process to produce acellular bio-scaffolds for ‘de novo’ regrowth or application as tissue ‘patches’ that compensate, e.g., large volumetric muscle loss in reconstructive surgery. Therefore, generating standardized acellular muscle scaffolds marks a pressing area of need. Although animal muscle decellularization protocols were established, those are mostly manually performed and lack defined bioreactor environments and metrologies to assess decellularization quality in real-time. To close this gap, we engineered an automated bioreactor system to provide chemical decellularization solutions to immersed whole rat gastrocnemius medialis muscle through perfusion of the main feeding arteries. Results: Perfusion control is adjustable according to decellularization quality feedback. This was assessed both from (i) ex situ assessment of sarcomeres/nuclei through multiphoton fluorescence and label-free Second Harmonic Generation microscopy and DNA quantification, along with (ii) in situ within the bioreactor environment assessment of the sample’s passive mechanical elasticity. Conclusion: We find DNA and sarcomere-free constructs after 72 h of 0.1% SDS perfusion-decellularization. Furthermore, passive elasticity can be implemented as additional online decellularization quality measure, noting a threefold elasticity decrease in acellular constructs. Significance: Our MyoBio represents a novel and useful automated bioreactor environment for standardized and controlled generation of acellular whole muscle scaffolds as a valuable source for regenerative medicine. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00189294
- Volume :
- 69
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- IEEE Transactions on Biomedical Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 157551694
- Full Text :
- https://doi.org/10.1109/TBME.2022.3142317