Back to Search
Start Over
A simple electrochemiluminesecence aptasenor using a GCE/NCQDs/aptamers for detection of Pb.
- Source :
- Environmental Technology; Jun2022, Vol. 43 Issue 15, p2270-2277, 8p
- Publication Year :
- 2022
-
Abstract
- An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb<superscript>2+</superscript> with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb<superscript>2+</superscript> aptamers were covalently bound to the NCQDs to form a stable GCE/NCQDs/aptamers. On addition of Pb<superscript>2+</superscript>, the chain aptamers change to a pb<superscript>2+</superscript> G-quadruplex conformation, which lead to a large decrease in the ECL intensity. The variation of intensity and the logarithm of the Pb<superscript>2+</superscript> concentration had a good linear relationship (R<superscript>2</superscript> = 0.998). The detection range was wide (50 pM to 387.9 nM) with a low detection limit (18.9 pM). In interference experiments, the ECL Pb<superscript>2+</superscript> aptasensor did not suffer from interference and it had good stability. The NCQDs ECL aptasensor can detect Pb<superscript>2+</superscript> quickly and accurately, and provides a fast and efficient method for detection of Pb<superscript>2+</superscript>. Compared with literatures, the Pb<superscript>2+</superscript> aptasensor has simpler preparation process, lower cost; furthermore, it is more environmentally friendly. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09593330
- Volume :
- 43
- Issue :
- 15
- Database :
- Complementary Index
- Journal :
- Environmental Technology
- Publication Type :
- Academic Journal
- Accession number :
- 157383501
- Full Text :
- https://doi.org/10.1080/09593330.2021.1871661