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Molecular Characterization of a New Ecotype of Holoparasitic Plant Orobanche L. on Host Weed Xanthium spinosum L.

Authors :
Yousefi, Ali Reza
Ahmadikhah, Asadollah
Fotovat, Reza
Rohani, Leila
Soheily, Foad
Uberti, Daniela Letizia
Mastinu, Andrea
Source :
Plants (2223-7747); Jun2022, Vol. 11 Issue 11, p1406-1406, 11p
Publication Year :
2022

Abstract

A species of Orobanche was observed on spiny cocklebur (Xanthium spinosum) for the first time in Iran and tentatively was named IR-Iso.This study was conducted to make a phylogenetic analysis of the Orobanche using 5.8S rRNA region sequences, and also to better understand its sequence pattern. The full-length ITS1-ITS2 region of the new Orobanche isolate was PCR-amplified from the holoparasitic plant parasitizing X. spinosum. Sequences of the amplicons from the isolate were 100% identical but differed by 5.6–6.7% from most homologous GenBank accessions to 37.9% divergence from distant species. The analysis of the molecular variance showed that variation between-population (61.9%, SE = 0.04) was larger than within-population. Neighbor-joining analysis placed the Iranian isolate in the same clade as most of the Orobanche and Phelipanche species. The isolate was more closely related to Orobanche aegyptiaca (from China), and this was confirmed by using a structure analysis. However, complementary analyses showed that the Iranian isolate has a unique nucleotide substitution pattern, and hence it was considered as an ecotype of O. aegyptiaca (ecotype Alborzica). In this paper we report on the association between this new ecotype of Orobanche and X. spinosum. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22237747
Volume :
11
Issue :
11
Database :
Complementary Index
Journal :
Plants (2223-7747)
Publication Type :
Academic Journal
Accession number :
157369128
Full Text :
https://doi.org/10.3390/plants11111406