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Differential display analysis of gene expression in yeast.
- Source :
- Cellular & Molecular Life Sciences; Aug2002, Vol. 59 Issue 8, p1241-1245, 05p, 1 Diagram, 3 Charts
- Publication Year :
- 2002
-
Abstract
- RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome and simultaneously provides valuable information towards further refinement of this technique. Several examples discussed below illustrate how DD coupled with classical yeast genetic approaches may be used for studying transcriptionally regulated genetic systems. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENE expression
YEAST
RNA
ELECTROPHORESIS
POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 1420682X
- Volume :
- 59
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Cellular & Molecular Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 15728536
- Full Text :
- https://doi.org/10.1007/s00018-002-8502-y