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Enhanced Immunogenicity of Inactivated Dengue Vaccines by Novel Polysaccharide-Based Adjuvants in Mice.
- Source :
- Microorganisms; May2022, Vol. 10 Issue 5, p1034-1034, 16p
- Publication Year :
- 2022
-
Abstract
- Dengue fever, caused by any of four dengue viruses (DENV1-4), is a major global burden. Currently, there is no effective vaccine that prevents infection in dengue naïve populations. We tested the ability of two novel adjuvants (Advax-PEI and Advax-2), using aluminum hydroxide (alum) as control, to enhance the immunogenicity of formalin- or psoralen-inactivated (PIV or PsIV) DENV2 vaccines in mice. Mice were vaccinated on days 0 and 30, and serum samples were collected on days 30, 60, 90, and 101. Neutralizing antibodies were determined by microneutralization (MN) assays, and the geometric mean 50% MN (MN<subscript>50</subscript>) titers were calculated. For the PIV groups, after one dose MN<subscript>50</subscript> titers were higher in the novel adjuvant groups compared to the alum control, while MN<subscript>50</subscript> titers were comparable between the adjuvant groups after the second dose. For the PsIV groups, both novel adjuvants induced higher MN<subscript>50</subscript> titers than the alum control after the second dose. Spleen cells were collected on days 45 and 101 for enzyme-linked immunospot (ELISPOT) for IFNγ and IL4. Both PIV and PsIV groups elicited different degrees of IFNγ and IL4 responses. Overall, Advax-2 gave the best responses just ahead of Advax-PEI. Given Advax-2's extensive human experience in other vaccine applications, it will be pursued for further development. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 10
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Microorganisms
- Publication Type :
- Academic Journal
- Accession number :
- 157241808
- Full Text :
- https://doi.org/10.3390/microorganisms10051034