Back to Search
Start Over
Chemical composition, antibiofilm, cytotoxic, and anti-acetylcholinesterase activities of Myrtus communis L. leaves essential oil.
- Source :
- BMC Complementary Medicine & Therapies; 5/20/2022, Vol. 22 Issue 1, p1-16, 16p, 5 Charts, 3 Graphs
- Publication Year :
- 2022
-
Abstract
- Background: The potential of essential oils (EOs) and of their principal constituents for eradication of biofilm and at the same time the research of new potential acetylcholinesterase inhibitors is gaining increasing interest in last years. The aims of this study were to determine the chemical composition and to evaluate the antibacterial, cytotoxic, and anti-acetylcholinesterase properties of Myrtus communis leaves essential oil and its main constituents. Methods: Essential oil was obtained by hydrodistillation of M. communis L. leaves and was analyzed by GC and GC–MS. The antimicrobial activity was carried out against both gram-negative and gram-positive bacteria. The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). Then, the capacity of essential oil and its main constituent to inhibit biofilm growth, with the method of O'Toole and Kolterand, and the metabolic activity of biofilm cells through the MTT colorimetric method were evaluated at different times. Moreover, was studied the potential cytotoxic activity against SH-SY5Y cell line with MTT assay and the anti-acetylcholinesterase activity using Ellman's assay. Results: Myrtenyl-acetate, 1,8 cineole, α-pinene, and linalool were the main components in the EO. The myrtle EO, at the minimum tested dose (0.4 mg/ml), inhibited S. aureus biofilm by 42.1% and was capable of inhibiting the biofilm cell metabolism in all tested strains, except Staphylococcus aureus. Moreover, the EO showed good cytotoxic and anti-acetylcholinesterase activities IC<subscript>50</subscript> of 209.1 and 32.8 μg/ml, respectively. Conclusions: The results suggest that myrtle EO and its main constituents could be used as possible products that could act against the resistant pathogenic species E. coli, P. aeruginosa, L. monocytogenes and S. aureus, on the other hand, as possible coadjutants in the treatment of neurological diseases. [ABSTRACT FROM AUTHOR]
- Subjects :
- MEDICINAL plants
ESSENTIAL oils
NEUROBLASTOMA
ANALYSIS of variance
GRAM-negative bacteria
CHOLINESTERASE inhibitors
BIOFILMS
ANTINEOPLASTIC agents
PHYTOCHEMICALS
GAS chromatography
LEAVES
MASS spectrometry
DESCRIPTIVE statistics
DOSE-effect relationship in pharmacology
STAPHYLOCOCCUS aureus
CELL lines
DATA analysis software
ANTIBIOTICS
GRAM-positive bacteria
CALORIMETRY
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 22
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 157006339
- Full Text :
- https://doi.org/10.1186/s12906-022-03583-4