Quality Standard of Rutin in the Leaves of Thespesia populnea.

Objectives] To establish a quality standard method for detecting rutin in leaves of Thespesia populnea. [Methods] The leaves of T. populnea as a reagent, the rutin in the leaves of ! populnea was extracted by alcohol extraction, and quantitatively analyzed by high performance liquid chromatography $ HPLC). [ Results] In the range of 0. 21 -3.36 xg, the peak area A of rutin in the leaves of ! populnea had a good linear relationship with its injection volume m. A = 1 561.6 m - 6. 893, and the correlation coefficient r = 1. 000 0. The method was applied to the determination of rutin ethyl acetate extract in the leaves of T. populnea, the average recovery rate was 101.75%, and the RRD was 1.32%. [ Conclusions] This method is simple and feasible, the results are accurate, the repeatability is good, and the separation efect is good. It is expected to provide a scientific idea for the determination of rutin in the leaves of T. populnea. [ABSTRACT FROM AUTHOR]