Study the Ability of Pseudomonas Aeruginosa Isolated from Different Clinical Cases to Biofilm Formation and Detection of Algd Gene.

98 samples were collected from various clinical sources included (Burns, wounds, urines, sputums, blood) From the city of Baghdad, After performing the biochemical and microscopic examination, 52 isolates were obtained for Pseudomonas aeruginosa, 17 (32.7 %) isolates from burn infection, 12 (23%) isolates from Wound infection 11 (21.2 %) isolates from urine infection, 7 (13.5 %) isolates of sputum and 5 (9.6 %) isolates from blood. Bacteria susceptibility to form biofilm has been detectedby microtiter plate method, The results showed that 80 % of the bacterial isolates were produced the biofilm with different proportions, alg D gene (alginate production) has been detected by polymerase chain reaction (PCR) Which plays an essential role in the formation of the biofilm, The PCR results showed that the percentage of gene presence was(95.4 %). [ABSTRACT FROM AUTHOR]