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Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A.
- Source :
- BMC Complementary Medicine & Therapies; 4/19/2022, Vol. 22 Issue 1, p1-12, 12p, 5 Graphs
- Publication Year :
- 2022
-
Abstract
- Background: Tanshinone I (Tan-I), an ingredient of Salvia miltiorrhiza, displays protective effects in several disease models. We aim to study the effect of Tan-I on renal fibrosis and explore its underlining mechanism. Methods: Rat renal fibroblasts (NRK-49F) were used as an in vitro model to study the effect of Tan-I. Mouse renal fibrosis model was induced by unilateral ureteral obstruction (UUO) or peritoneally injection of aristolochic acid I (AAI). Results: We found that Tan-I dose-dependently inhibited the expression of pro-fibrotic markers in rat renal fibroblasts. Masson staining and Western blotting analysis showed that Tan-I treatment attenuated renal fibrosis in UUO or AAI induced fibrotic kidneys. RNA sequencing analysis identified inhibin beta-A (INHBA), a ligand of TGF-β superfamily, as a downstream target of Tan-I in fibrotic kidneys, which were further verified by qPCR. Western blotting analysis showed that INHBA is up-regulated in UUO or AAI induced fibrotic kidneys and Tan-I reduced the expression of INHBA in fibrotic kidneys. Inhibition of INHBA by Tan-I was further confirmed in rat fibroblasts. Moreover, knockdown of INHBA reduced the expression of pro-fibrotic markers and abolished the ani-fibrotic effect of Tan-I in rat renal fibroblasts. Conclusions: We conclude that Tan-I attenuates fibrosis in fibrotic kidneys through inhibition of INHBA. [ABSTRACT FROM AUTHOR]
- Subjects :
- KIDNEY disease prevention
IN vitro studies
BIOMARKERS
TRANSFORMING growth factors-beta
MEDICINAL plants
FIBROBLASTS
STAINS & staining (Microscopy)
SEQUENCE analysis
CELL culture
IN vivo studies
ANIMAL experimentation
WESTERN immunoblotting
FIBROSIS
RATS
GENE expression
GLYCOPROTEINS
RESEARCH funding
PLANT extracts
PEPTIDE hormones
POLYMERASE chain reaction
DATA analysis software
RNA probes
MICE
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 22
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 156400126
- Full Text :
- https://doi.org/10.1186/s12906-022-03592-3