カキのノロウイルス定量におけるRNA抽出機器および ワンステップRT-PCR試薬の比較

While exports of oysters from Japan are increasing in recent years, it is necessary to establish a pre-export testing implementation system in Japan using ISO 15216 method, which is used internationally as a testing method for norovirus in oyster. However, NucliSENS miniMAG (bio-Merieux), an RNA extraction device widely used in Europe, has already been discontinued and is not available in Japan. In this study, we verified the substitutability of Mealstrom 8 (TANBead) as an alternative suggested in a previous report, using pacific oyster (Crassostrea gigas), which is commonly consumed in Japan. Moreover, we verified the substitutability of the three reagent kits available in Japan for RNA UltraSense one-step quantitative RT-PCR system (Thermo Fisher Scientific), which is the reagent recommended by the o伍 cial protocol of ISO 15216 method. As a result, viral RNA was extracted more efficiently by Maelstrom 8 than by the NucliSENS miniMAG. And of the three reagent kits, CEERAMTOOLS noroGI-GII@ceeramTools (bioMerieux) was shown to have the same performance of gene detection as UltraSense. These results are expected to contribute to establishment of the pre-export testing system for oysters using ISO 15216 method in Japan. [ABSTRACT FROM AUTHOR]