Back to Search
Start Over
Characterization of the Spike Glycoprotein and Construction of an Epitope-Based Vaccine Candidate against Indonesian SARS-CoV-2: In Silico Study.
- Source :
- Systematic Reviews in Pharmacy; 2020, Vol. 11 Issue 7, p404-413, 10p
- Publication Year :
- 2020
-
Abstract
- Introduction: SARS-CoV-2, a new member of the coronavirus family that originated from Wuhan, China, is the agent of COVID-19 pandemic and has rapidly spread globally. Objective: We characterized the spike (S) glycoprotein gene from the Indonesian SARS-CoV-2 isolates to investigate its genetic composition, predict the B cell epitopes, and construct the molecular phylogenetic among Indonesian SARS-CoV-2 isolates. Methods: We employed Wuhan-Hu-1 isolate available in GenBank, NCBI and fourteen Indonesian SARS-CoV-2 isolates acquired from the database (GISAID EpiCoV). We performed using the MEGA X for genetic and amino acid mutations and construct molecular phylogenetic tree. We used IEDB web server to predict epitopes, evaluated allergenicity by applying AllerTOP v.2.0 web server, and non-toxic antigens applying the ToxinPred web server. Results: Interestingly, we discovered that the Indonesian SARS-CoV-2 isolates genetic composition do not have significant changes compared with the reference sequence based on the S glycoprotein gene. In addition, we proposed NSASFSTFKCYGVSPTKLNDLCFTNV as a candidate for a peptide-based vaccine against COVID-19. Furthermore, we also presented the molecular phylogenetic of Indonesian SARS-CoV-2 isolates and other coronaviruses. Conclusion: In summary, this study supplied data regarding mutations in the S glycoprotein and we proposed a candidate for peptide-based vaccine against COVID-19. However, this research still requires further genetic analysis and we recommend improvement in the molecular epidemiological surveillance on COVID-19 in Indonesia. [ABSTRACT FROM AUTHOR]
- Subjects :
- SARS-CoV-2
INTERNET servers
B cells
COVID-19 pandemic
Subjects
Details
- Language :
- English
- ISSN :
- 09758453
- Volume :
- 11
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Systematic Reviews in Pharmacy
- Publication Type :
- Academic Journal
- Accession number :
- 156321749