Matrix Metalloproteinase-1 (MMP-1) ExpressionandDensity of Collagen Fibers following Applicationof HaruanFish (Channa striata) Extract in Inflamed Pulpof WistarRat.

Pulp perforation in teeth with reversible pulpitis can occur due to iatrogenic factors. This condition can be treated with direct pulp capping using calcium hydroxide. Still, this material can cause necrosis of the superficial layer of the pulp and dentin, which produces tunnel defects in the long term. Therefore, natural ingredients of fish extract containing essential compounds for the process of tissue syntheses such as albumin, amino acids, and several minerals that can play a role in accelerating the wound healing process is being developed. This study was conducted to determine the effect of the application of Haruan fish extracts on MMP-1 expression and the density of collagen fibers in the dental pulp of inflamed Wistar rats. A total of 27 samples were divided into three groups: the negative control group, treatment (extract of Haruan fish), and positive control (calcium hydroxide). The test material was applied to class 1 cavities, made to reach the pulp, and given LPS e-coli then left for 3 hours. Samples were decapitated on 3 rd,7 th and 14 th days. Data were analyzed using the Anova and Chi- Square test. Based on the study, there was a decrease in MMP-1 expression on 3 rd,7 th and 14 th days, but there was no statistically significant difference. The density of collagen fibers was seen as thicker scores on the 7th day, but there was no significant difference in the time of observation. Based on the conducted study, Haruan fish extract has been shown to reduce MMP-1 expression in inflamed pulp and trigger the proliferation of fibroblast cells to increase the density of collagen fibers. [ABSTRACT FROM AUTHOR]