A fluorescence nanoplatform for the determination of hydrogen peroxide and adenosine triphosphate via tuning of the peroxidase-like activity of CuO nanoparticle decorated UiO-66.

A novel nanocomposite of CuO nanoparticle-modified Zr-MOF (CuO/UiO-66) was synthesized and developed as a fluorescence nanoplatform for H₂O₂ and adenosine triphosphate (ATP) via the "turn-on–off" mode in the presence of terephthalic acid (TA). The structure of CuO/UiO-66 was thoroughly characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and other techniques. The CuO/UiO-66 with enhanced peroxidase-like (POD) activity obtained due to the Zr⁴⁺ in UiO-66 is beneficial to the aggregation of CuO NPs on its surface. As a result, the strengthened fluorescence at 425 nm with the excitation of 300 nm was found due to the highly fluorescent species of TAOH. This is produced by the oxidation of TA by ·OH that came from the catalysis of H₂O₂ via the peroxidase mimic of CuO/UiO-66. Hence the modification of CuO NPs on porous UiO-66 can provide a friendly and sensitive physiological condition for H₂O₂ detection. However, upon addition of ATP, the fluorescence intensity of TAOH at 425 nm effectively declined owing to the formation of complexation of Zr⁴⁺-ATP and the interaction of CuO to ATP which hampers the catalytic reaction of CuO/UiO-66 to H₂O₂. The specific interaction induced "inhibition of the peroxide-like activity" endows the sensitive and selective recognition of ATP. The detection limits were 16.87 ± 0.2 nM and 0.82 ± 0.1 nM, and linear analytical ranges were 0.02–100 μM and 0.002–30 μM for H₂O₂ and ATP, respectively. The novel strategy was successfully applied to H₂O₂ and ATP determination in serum samples with recoveries of 97.2–103.8% for H₂O₂ and 97.6–101.7% for ATP, enriching the avenue to design functional MOFs and providing new avenue of multicomponent bioanalysis. [ABSTRACT FROM AUTHOR]