Transcriptome sequencing analysis of dwarfing traits in Brassica napus line DW871.

DW871 is a Brassica napus dwarf line with unusual and excellent plant type. To study the dwarf-related genes and their expression patterns of DW871. we took B. napus HW871 as control and selected stem tissues at the bolting stage for transcriptome gene expression analysis. Results showed that there were 8665 significant differentially expressed genes between DW871 and HW881. of which 2582 were up-regulated and 6083 down-regulated. The enrichment analysis of GO could be annotated to 22 subcategories in 3 categories. the enrichment analysis of KEGG was annotated to 295 pathways in 31 subcategories in 5 categories. We screened genes closely related to dwarfing traits from significantly different genes, 43 in pectin degradation, 69 in auxin signal transduction, and annotated 22 in the monolignol biosynthesis. It was found that the dwarfing character of DW871 was related to cell elongation, abnormal cell wall formation and cell division, and obstruction of auxin signal transduction. In particular, in monolignol biosynthesis, it is possible to regulate a series of physiological and biochemical processes related to lignin by regulating the expression of enzymes such as PAL and CCR. The differential genes related to dwarfing traits were screened in this research. It could be hypothesized that the dwarfing mechanism of DW871 involving abnormal auxin response and lignin synthesis, which lead to abnormal cell elongation, growth and cell wall changes, and ultimately lead to the dwarfing of DW871. [ABSTRACT FROM AUTHOR]