Back to Search
Start Over
The voltage-gated K+ channel Kv1.3 modulates platelet motility and α2β1 integrin-dependent adhesion to collagen.
The voltage-gated K+ channel Kv1.3 modulates platelet motility and α2β1 integrin-dependent adhesion to collagen.
- Source :
- Platelets; 2022, Vol. 33 Issue 3, p451-461, 11p
- Publication Year :
- 2022
-
Abstract
- Kv1.3 is a voltage-gated K<superscript>+</superscript>-selective channel with roles in immunity, insulin-sensitivity, neuronal excitability and olfaction. Despite being one of the largest ionic conductances of the platelet surface membrane, its contribution to platelet function is poorly understood. Here we show that Kv1.3-deficient platelets display enhanced ADP-evoked platelet aggregation and secretion, and an increased surface expression of platelet integrin α<subscript>IIb</subscript>. In contrast, platelet adhesion and thrombus formation in vitro under arterial shear conditions on surfaces coated with collagen were reduced for samples from Kv1.3<superscript>−/-</superscript> compared to wild type mice. Use of collagen-mimetic peptides revealed a specific defect in the engagement with α<subscript>2</subscript>β<subscript>1</subscript>. Kv1.3<superscript>−/-</superscript> platelets developed significantly fewer, and shorter, filopodia than wild type platelets during adhesion to collagen fibrils. Kv1.3<superscript>−/-</superscript> mice displayed no significant difference in thrombus formation within cremaster muscle arterioles using a laser-induced injury model, thus other pro-thrombotic pathways compensate in vivo for the adhesion defect observed in vitro. This may include the increased platelet counts of Kv1.3<superscript>−/-</superscript> mice, due in part to a prolonged lifespan. The ability of Kv1.3 to modulate integrin-dependent platelet adhesion has important implications for understanding its contribution to normal physiological platelet function in addition to its reported roles in auto-immune diseases and thromboinflammatory models of stroke. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09537104
- Volume :
- 33
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Platelets
- Publication Type :
- Academic Journal
- Accession number :
- 155780855
- Full Text :
- https://doi.org/10.1080/09537104.2021.1942818