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Prenylcysteine Oxidase 1 (PCYOX1), a New Player in Thrombosis.
- Source :
- International Journal of Molecular Sciences; Mar2022, Vol. 23 Issue 5, p2831, 1p
- Publication Year :
- 2022
-
Abstract
- Prenylcysteine Oxidase 1 (PCYOX1) is an enzyme involved in the degradation of prenylated proteins. It is expressed in different tissues including vascular and blood cells. We recently showed that the secretome from Pcyox1-silenced cells reduced platelet adhesion both to fibrinogen and endothelial cells, suggesting a potential contribution of PCYOX1 into thrombus formation. Here, we show that in vivo thrombus formation after FeCl<subscript>3</subscript> injury of the carotid artery was delayed in Pcyox1<superscript>−/−</superscript> mice, which were also protected from collagen/epinephrine induced thromboembolism. The Pcyox1<superscript>−/−</superscript> mice displayed normal blood cells count, vascular procoagulant activity and plasma fibrinogen levels. Deletion of Pcyox1 reduced the platelet/leukocyte aggregates in whole blood, as well as the platelet aggregation, the alpha granules release, and the α<subscript>IIb</subscript>β<subscript>3</subscript> integrin activation in platelet-rich plasma, in response to adenosine diphosphate (ADP) or thrombin receptor agonist peptide (TRAP). Washed platelets from the Pcyox1<superscript>−/−</superscript> and WT animals showed similar phosphorylation pathway activation, adhesion ability and aggregation. The presence of Pcyox1<superscript>−/−</superscript> plasma impaired agonist-induced WT platelet aggregation. Our findings show that the absence of PCYOX1 results in platelet hypo-reactivity and impaired arterial thrombosis, and indicates that PCYOX1 could be a novel target for antithrombotic drugs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 23
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 155705025
- Full Text :
- https://doi.org/10.3390/ijms23052831