Comparative Study of Three Raspberry Cultivar (Rubus idaeus L.) Leaves Metabolites: Metabolome Profiling and Antioxidant Activities.

Raspberry (Rubus idaeus L.), known as one of the famous healthy fruits an d are consumed fresh or processed products all over the world. The antioxidation activity of raspberry fruits as well as leaves have been widely investigated. To better understand the metabolite accumulation mechanisms and to develop different functional cultivars, we performed a non-targeted metabolomics analysis using LC-MS/MS to investigate the contents of existing components from three raspberry cultivars, Autumn Britten, Autumn Bliss, and Red Autumn leaves, respectively. The results show multiple differentially accumulated metabolites among three cultivars, especially for the lipids (α-linolenic acid and eicosatetraenoic acid), amino acids and their derivatives (L-cysteine, Phenylalanine), flavonoids (Kaempferol 3-O-rhamnoside-7-O-glucoside, Quercetin 3-glucoside), and vitamins (Biotin, Thiamine, Vitamin K2), etc. The in vitro cellular antioxidant activities of three raspberry cultivars leaves ethanol extracts (RLEE) were also characterized. Through comparison the superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and reactive oxygen species (ROS) levels before or after RLEE protection of L929 fibroblast cells upon excessive UVB exposure, we evaluated the antioxidation potentials for all three cultivar RLEEs. It turns out the raspberry Autumn Britten leaf extract holds the greatest potential for protecting the L929 fibroblast cells from UVB induced damage. Our study provides theoretical support for screening of active metabolites from three raspberry cultivars leaves, spanning metabolites' accumulation to cell damage protection, which could be used to refine bioactivity assessment for different raspberry cultivars suitable for antioxidant products extraction. [ABSTRACT FROM AUTHOR]