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Identification of essential genes involved in metabolism‐based resistance mechanism to fenoxaprop‐P‐ethyl in Polypogon fugax.

Authors :
Zhao, Ning
Yang, Jiajia
Jiang, Minghao
Liao, Min
Cao, Haiqun
Source :
Pest Management Science; Mar2022, Vol. 78 Issue 3, p1164-1175, 12p
Publication Year :
2022

Abstract

BACKGROUND Metabolic resistance is a worldwide concern for weed control but has not yet been well‐characterized at the genetic level. Previously, we have identified an Asia minor bluegrass (Polypogon fugax Nees ex Steud.) population AHHY exhibiting cytochrome P450 (P450)‐involved metabolic resistance to fenoxaprop‐P‐ethyl. In this study, we aimed to confirm the metabolic fenoxaprop‐P‐ethyl resistance in AHHY and uncover the potential herbicide metabolism‐related genes in this economically damaging weed species. RESULTS: Liquid chromatography–tandem mass spectrometry (LC–MS/MS) assays indicated the metabolic rates of fenoxaprop‐P‐ethyl were significantly faster in resistant (R, AHHY) than in susceptible (S, SDTS) plants. The amount of phytotoxic fenoxaprop‐P peaked at 12 h after herbicide treatment (HAT) and started to decrease at 24 HAT in both biotypes. R and S plants at 24 HAT were sampled to conduct isoform‐sequencing (Iso‐Seq) and RNA‐sequencing (RNA‐Seq). A reference transcriptome containing 24 972 full‐length isoforms was obtained, of which 24 329 unigenes were successfully annotated. Transcriptomic profiling identified 28 detoxifying enzyme genes constitutively and/or herbicide‐induced up‐regulated in R than in S plants. Real‐time quantitative polymerase chain reaction (RT‐qPCR) confirmed 17 genes were consistently up‐regulated in R and its F1 generation plants. They were selected as potential fenoxaprop‐P‐ethyl metabolism‐related genes, including ten P450s, one glutathione‐S‐transferase, one UDP‐glucosyltransferase, and five adenosine triphosphate (ATP)‐binding cassette transporters. CONCLUSION: This study revealed that the enhanced rates of fenoxaprop‐P‐ethyl metabolism in P. fugax were very likely driven by the herbicide metabolism‐related genes. The transcriptome data generated by Iso‐Seq combined with RNA‐Seq will provide abundant gene resources for understanding the molecular mechanisms of resistance in P. fugax. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1526498X
Volume :
78
Issue :
3
Database :
Complementary Index
Journal :
Pest Management Science
Publication Type :
Academic Journal
Accession number :
155147315
Full Text :
https://doi.org/10.1002/ps.6733