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Antileishmanial, antibacterial and cytotoxicity activity of the extracts, fractions, and compounds from the fruits and stem bark extracts of Pentadesma butyracea Sabine.

Authors :
Garba, Jean Koffi
Nguengang, Ruland Tchuinkeu
Youmbi, Gwladys Tatiana
Menatche, Joel Njopnu
Ngansop, Cyrille Armel Njanpa
Bankeu, Jean Jules Kezetas
Chouna, Jean Rodolphe
Boyom, Fabrice Fekam
Sewald, Norbert
Lenta, Bruno Ndjakou
Source :
Zeitschrift für Naturforschung B: A Journal of Chemical Sciences; Jan2022, Vol. 77 Issue 1, p9-15, 7p
Publication Year :
2022

Abstract

The search for antileishmanial plants used in traditional medicine led to the choice of CH<subscript>2</subscript>Cl<subscript>2</subscript>–MeOH (1:1) crude extract of the fruits and stem bark of Pentadesma butyracea Sabine (Clusiaceae) which displayed good activity in vitro against Leishmania donovani 1S (MHOM/SD/62/1S) promastigotes during preliminary screening with IC<subscript>50</subscript> values 5.96 and 26.43 μg mL<superscript>−1</superscript>, respectively. The fractionation of both extracts using flash chromatography yielded active fractions with IC<subscript>50</subscript> values ranging from 2.71 to 18.88 μg mL<superscript>−1</superscript>. Fourteen compounds (1–14) were isolated from the obtained fractions using successive column chromatographies and their structures were elucidated based on the analysis of their NMR and MS data. Daphnifolin (1), epicathechin (3), α-mangostin (9) and 9-hydroxycalabaxanthone (14) exhibited potent antileismanial activity against L. donovani 1S (MHOM/SD/62/1S) promastigotes with IC<subscript>50</subscript> values of 2.01, 9.09, 3.37, and 6.87 μg mL<superscript>−1</superscript>, respectively and good selectivity towards Raw 264.7 macrophage cells (SI > 2.4). Extracts, fractions and some isolates were also assessed in vitro for their antibacterial activity against six bacterial strains [Salmonella typhi (CPC), Enterobacter cloacae (CPC), Pseudomonas aeruginosa HM801, Staphylococcus aureus ATCC 43300, Streptococcus pneumoniae ATCC 491619, Escherichia coli ATCC 25322] using serial microdilution method. Among the tested samples, the stem bark extract of P. butyracea as well as compounds 2 and 8 showed good to moderate activity against the aforementioned bacterial strains with MIC ≤ 250 μg mL<superscript>−1</superscript>. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09320776
Volume :
77
Issue :
1
Database :
Complementary Index
Journal :
Zeitschrift für Naturforschung B: A Journal of Chemical Sciences
Publication Type :
Academic Journal
Accession number :
154831026
Full Text :
https://doi.org/10.1515/znb-2021-0077