THE RESPONSE OF FOREIGN BODY GIANT CELLS IN THE IMPLANTATION OF DEMINERALIZED DENTINE MATERIAL MEMBRANE AS A GUIDED BONE REGENERATION.

The failure or delayed healing process may have occurred in critical size defect condition. Non-osteogenic cell migration in critical size defect can be prevented by aapplication of a guided bone regeneration (GBR). GBR may serve as osteoinductive biomaterial, one of example of GBR is dentine bovine. Demineralized Dentine Material Membrane (DDMM) can be obtained from bovine dentine as GBR. Thus, the aim of this study is to investigate the Demineralized Dentine Membrane Material (DDMM) as a Guided Bone Regeneration (GBR) effect on foreign body giant cell. The sample consisted of sixty, 2-3 months old male rats of Wistar rats (Rattus novergicus) with 250-300 grams body weight. The samples were divided into two control groups (K) and three treatment groups (P). Mandibular bone defect with sized 5 x 5 mm in group K(-) was not applied with GBR membrane, while Group K(+) was applied with Bovine Pericardium Collagen Membrane (BPCM). In the treatment groups, DDMM was applied in Group P1, DDMM and grafts were applied in Group P2, BPCM and grafts were induced in Group P3. Six samples were sacrificed on Day 3 and 7 post-operation for the histology examination using Hematoxylin Eosin (HE) staining. Foreign body giant cells counts were examined and statistically analyzed using an independent t-test (p<0.05). As the results, there was no significant difference in the amount of giant cell in every groups on Day 3 and 7. Meanwhile, DDMM showed the comparable effect on giant cell of mandibular critical size defect to BPCM. In conclusion, indeed, the implantation of DDMM has potential for being used as an alternative membrane for absorbable guided bone regeneration biomaterial. [ABSTRACT FROM AUTHOR]