Biotin interference: evaluation of an updated thyroglobulin electrochemiluminescent immunoassay.

Keywords: biotin; ECLIA; immunoassay; method evaluation; thyroglobulin EN biotin ECLIA immunoassay method evaluation thyroglobulin e46 e49 4 01/07/22 20220115 NES 220115 To the Editor, Biotin interference in immunoassays is well-known and researched [[1]]. Exogenous biotin disturbs this interaction, potentially causing falsely decreased results for sandwich immunoassays, such as the Elecsys SP ® sp thyroglobulin (Tg) assay (Roche Diagnostics, Switzerland) [[4]]. Plasma peak concentrations induced by OTC supplements (53-141 ng/mL) or by treatment with high biotin doses (up to 1,160 ng/mL plasma levels) do not exceed the biotin threshold of the Tg2New assay insert or our experimentally confirmed threshold [[1]]. [Extracted from the article]