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Silver Ion-Complexation High-Speed Countercurrent Chromatography Coupled with Prep-HPLC for Separation of Sesquiterpenoids from Germacrene A Fermentation Broth.

Authors :
Huanzhu Zhao
Xiangyun Song
Ali, Iftikhar
Hussain, Manzoor
Mehmood, Andleeb
Siyo, Baraa
Qianshan Gao
Li Cui
Aziz, Shahid
Hussain, Hidayat
Wenya Ma
Dawei Qin
Daijie Wang
Source :
Fermentation (Basel); Dec2021, Vol. 7 Issue 4, p1-11, 11p
Publication Year :
2021

Abstract

A silver ion high-speed counter-current chromatography ([Ag+]-HSCCC) was developed to separate and purify five sesquiterpenoids from germacrene A fermentation broth. The solvent system was consisted of n-hexane-methanol-silver nitrate (3 mol/L) solution (10:9.5:0.5, v/v). By employing this chromatographic protocol, five sesquiterpenoids named β-elemene (1; 54.1 mg), germacrene A (2; 28.5 mg), γ-selinene (3; 4.6 mg), β-selinene (4; 3.4 mg), and α-selinene (5; 1.3 mg) were obtained successfully from 500 mg extracted crude sample with purities of 97.1%, 95.2%, 98.2%, 96.3% and 98.5%, respectively, combined with preparative HPLC. The results reveal that the addition of metal ion in biphasic solvent system significantly improved the HSCCC separation factor of sesquiterpenoids. Meanwhile, our study also provided an alternate approach to separate the compounds with less polarity, also geometrical isomers and various natural product classes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23115637
Volume :
7
Issue :
4
Database :
Complementary Index
Journal :
Fermentation (Basel)
Publication Type :
Academic Journal
Accession number :
154406060
Full Text :
https://doi.org/10.3390/fermentation7040230