Intein-Mediated Protein trans-Splicing of the Recombinant Streptavidin on Magnetosomes.

When expressing streptavidin recombinant polypeptide on magnetosomes (called bacterial magnetic nanoparticles, or BMPs), the presence of endogenous bacterial biotin might be detrimental. In the study, the streptavidin monomer fragment (SA_1–116) was fused with the intein N-terminal (termed precursor SA_1–116-IN), and SA_1–116-IN was expressed in E. coli (BL21). Meanwhile, the SA_117–160 fragment was fused with the C-terminal intein, and then this chimeric polypeptide was expressed on magnetosomes by fusion with magnetosome membrance protein MamF. In the in vitro protein splicing system, the purified engineered magnetosomes (BMP-SA_117–160-IC) and the SA_1–116-IN precursor were mixed. Intein-mediated trans-splicing reaction was induced to produce the functional magnetic beads BMP-SA. Our results indicate that intein-mediated protein trans-splicing may lead to efficient synthesis of the recombinant streptavidin on the magnetosomes, showing its promising potential to produce other functional magnetic nanoparticles. [ABSTRACT FROM AUTHOR]