The reduction effect and mechanism of Deinococcus radiodurans transformed dsrA gene to uranyl ions.

Deinococcus radiodurans (DR) is highly resistant to ionizing radiation. This study aims to convert dsrA gene into DR to construct radiation-resistant genetically engineered bacteria (Deino-dsrA) with high reducibility, so as to enhance the reducing and enrichment ability of DR. Methods: the recombinant vector pRADK-dsrA was extracted and transformed into DR. 1. Radiation resistant gene engineering bacteria containing dsrA gene were constructed successfully. 2. In the most favorable conditions,contributing to approximately 92.45% U (VI) was removed. 3. Autioxidant enzyme activities in Deino-dsrA is higher than in Deino-pRADK, excepting the content of malondialdehyde. The uranium enrichment ability of the Deino-dsrA is better than the wild DR, and dsrA gene can increase its antioxidation capability by increasing the activity of oxidase. [ABSTRACT FROM AUTHOR]