Lipoteichoic Acid as Antibiofilm against Staphylococcus aureus.

Background: It is a polysaccharide substance produced by a group of microorganisms that helps it adhere to living and non-living surfaces and it is responsible for many diseases, as it has a role in staphylococcal diseases such as pneumonia, urinary tract infection and endocarditis. Although lipoteichoic acid is a component of biofilm. However, it has a role in preventing biofilm formation Materials and Methods: The methods of this study included isolated and identification of bacteria from UTI samples and identified by morphology characters and biochemical test to identify S.aureus. Then discover their ability to formbiofilm and Use lipoteichoic acid in different concentration as antibiofilm Results: The results of identification of bacterial isolatesshowed the colonies grown on blood agar causing the typical B hemolytic state, these samples not grown on MacConkey agar and under the microscope showed gram positive cocci, a cluster like grape, these samples were identified as Staphylococcus when grown on mannitol medium converted it to yellowish color and appeared round, smooth, raised, mucous and gelatinous. The staphylococcusaureus formed biofilms with different concentrations, where the fourth isolate was the lowest percentage (0.065), while the second and fifth isolates were given (0.120 and 0.153) and the first isolation was given (0.220), however the highest percentage was given by the third isolation (0.357). So this study showed that the concentrations of LTA (200) µg/mlare MIC. The results of this work showed that after 24 hours of treatment with LTA it impeded the formation of biofilms of Staphylococcus aureus and the result after treatment was the concentration 50 and 100µg/ml gave less percentage of inhibition, while 500µg/ml appeared 100% of inhibition, so (200,300 and 400 gave 61%, 80% and90% inhibition to biofilm respectively. [ABSTRACT FROM AUTHOR]